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In vivo immune modulation evaluation. (A) Immunofluorescence staining of C-C motif chemokine receptor 7 (CCR7, M1, green) and mannose receptor <t>(CD206,</t> M2, red). (B) Quantitative analysis of CCR7 and CD206 expression. Data are presented as mean ± SD ( n = 3). * indicates significant differences (* P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001).
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In vivo immune modulation evaluation. (A) Immunofluorescence staining of C-C motif chemokine receptor 7 (CCR7, M1, green) and mannose receptor <t>(CD206,</t> M2, red). (B) Quantitative analysis of CCR7 and CD206 expression. Data are presented as mean ± SD ( n = 3). * indicates significant differences (* P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001).
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In vivo immune modulation evaluation. (A) Immunofluorescence staining of C-C motif chemokine receptor 7 (CCR7, M1, green) and mannose receptor (CD206, M2, red). (B) Quantitative analysis of CCR7 and CD206 expression. Data are presented as mean ± SD ( n = 3). * indicates significant differences (* P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001).

Journal: Biomaterials Research

Article Title: Biomimetic Gradient Microporous Scaffold with a Triply Periodic Minimal Surface Enhances Osseointegration by Modulating Macrophage Polarization

doi: 10.34133/bmr.0266

Figure Lengend Snippet: In vivo immune modulation evaluation. (A) Immunofluorescence staining of C-C motif chemokine receptor 7 (CCR7, M1, green) and mannose receptor (CD206, M2, red). (B) Quantitative analysis of CCR7 and CD206 expression. Data are presented as mean ± SD ( n = 3). * indicates significant differences (* P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001).

Article Snippet: Rabbit anti-C-C motif chemokine receptor 7 (anti-CCR7) antibody (M1 marker, Boster, China) and rabbit anti-mannose receptor (anti-CD206) antibody (M2 marker, Boster, China) were used to stain the samples, as these are well-known markers for M1 and M2 macrophage phenotypes, respectively.

Techniques: In Vivo, Immunofluorescence, Staining, Expressing